Label-free miniaturized electrochemical nanobiosensor triaging platform for swift identification of the bacterial type

Gangwar, Rahul and Rao, Karri Trinadha and Khatun, Sajmina and Rengan, Aravind Kumar and Subrahmanyam, Challapalli and Vanjari, Siva Rama Krishna (2022) Label-free miniaturized electrochemical nanobiosensor triaging platform for swift identification of the bacterial type. Analytica Chimica Acta, 1233. pp. 1-10. ISSN 0003-2670

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Abstract

In chronic wounds, rapid identification of the bacterial type is critical for immediate clinical assessment. A novel, cost-effective, and label-free electrochemical nanobiosensor was developed with the help of an indigenously fabricated carbon paste working electrode to rapidly identify the bacterial type. The proposed platform made use of gold nanoparticles (AuNPs) to boost electrochemical activity, and the strong affinity of boronic acid moieties for diols allowed for detection and differentiation of gram + ve and gram -ve bacteria on the same platform. A scalable and robust miniaturized Electrochemical Cell (E-Cell) designed for the developed electrodes assisted in reducing sample waste, detection time, and Limit of Detection (LOD). Within 15 min, the proposed nano biosensing platform identified Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) bacteria with an excellent recovery rate for the blind samples. Because of its size and the extra lipopolysaccharides (LPS) layer containing diols, the bioelectrode demonstrated a superior response to E. coli, effectively distinguishing it from S. aureus. Furthermore, the proposed biosensing platform demonstrated an excellent shelf-life and reproducibility with acceptable selectivity and exhibited an excellent specificity towards bacteria, making it an ideal candidate for rapid identification of the bacterial type. © 2022 Elsevier B.V.

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IITH Creators:

IITH Creators	ORCiD
Rengan, Aravind Kumar	https://orcid.org/0000-0003-3994-6760
Subrahmanyam, Challapalli	https://orcid.org/0000-0002-2643-3854
Vanjari, Siva Rama Krishna	UNSPECIFIED

Item Type:

Article

Additional Information:

The authors acknowledge the Ministry of Education , India for funding the research.

Uncontrolled Keywords:

Bacterial identification; Carbon paste electrode; Electrochemical sensors; Escherichia coli; Gold nanoparticles; Staphylococcus aureus

Subjects:

Electrical Engineering
Biomedical Engineering
Chemistry

Divisions:

Department of Biomedical Engineering
Department of Chemistry
Department of Electrical Engineering

Depositing User:

. LibTrainee 2021