Screening and Absolute Quantification of a β-lactamase Resistance Gene NDM-1 in Lake Sediment

Ranjan, R. and Thatikonda, Shashidhar (2022) Screening and Absolute Quantification of a β-lactamase Resistance Gene NDM-1 in Lake Sediment. Pollution, 8 (3). pp. 729-739. ISSN 2383-451X

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Abstract

New Delhi Metallo-β-lactamase-1(NDM-1) is an enzyme that hydrolyzes a wide range of β-lactams antibiotics, including carbapenems. The presence of the NDM-1 inhibits the potential of β-lactam antibiotics in treating infections caused by bacterial strains carrying such resistances, thus leaving minimal treatment options available. Due to this, the rapid distribution of NDM-1 harboring bacteria accounts for a significant public health menace worldwide. These bacteria have been detected in clinical specimens and environmental compartments where bacterial infections are ubiquitous. In this study, identification and absolute quantification of NDM-1 in sixteen lake sediment samples collected in and around Hyderabad, India, was carried out using a real-time quantitative polymerase chain reaction (qPCR), and results were expressed in gene copy number/ng (nanogram) of template DNA. Thirteen samples (out of sixteen) displayed a positive signal for NDM-1 during the qPCR analysis with the highest gene copy number/ng of template DNA (71.8) being observed in the Amberpet STP. Three samples, samples those from Durgamcheru lake, Kandi lake, and Singur dam, were negative for the NDM-1 during the qPCR analysis. Hierarchical clustering analysis was performed to categorize the sampling location into different clusters based on pollution sources and the observed results were expressed in the form of a dendrogram. © 2022 University of Tehran. All rights reserved.

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IITH Creators:	IITH Creators ORCiD Thatikonda, Shashidhar https://orcid.org/0000-0003-2973-2224
Item Type:	Article
Additional Information:	The present research has been financially supported by University Grant Commission (UGC), India [Grant No: UGC-Ref. No.: 24625/ (NET-DEC. 2013)].
Uncontrolled Keywords:	Antibiotics resistance gene (ARG), Hierarchical clustering, NDM-1, qPCR, Sediment
Subjects:	Civil Engineering
Divisions:	Department of Civil Engineering
Depositing User:	. LibTrainee 2021
Date Deposited:	17 Oct 2022 05:12
Last Modified:	17 Oct 2022 05:12
URI:	http://raiithold.iith.ac.in/id/eprint/10969
Publisher URL:	https://doi.org/10.22059/poll.2022.327427.1140
Related URLs:	Author Google Scholar

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