Design and synthesis of spirooxindole–pyrrolidines embedded with indole and pyridine heterocycles by multicomponent reaction: anticancer and in silico studies

Mayakrishnan, Sivakalai and Kathirvelan, Devarajan and Arun, Yuvaraj and et al, . (2022) Design and synthesis of spirooxindole–pyrrolidines embedded with indole and pyridine heterocycles by multicomponent reaction: anticancer and in silico studies. New Journal of Chemistry, 46 (21). pp. 10089-10106. ISSN 1144-0546

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Abstract

Owing to the downsides of existing anticancer drugs, it is necessary to find more effective and selective anticancer agents for researchers in medicinal chemistry worldwide. Spirooxindoles are poised as privileged scaffolds because they exist in many natural products and bioactive molecules. Herein, we report an efficient, environment-friendly route for synthesizing a series of spirooxindoles using the 1,3-dipolar cycloaddition reaction of a dipolarophile with in situ generated azomethine ylide using ethanol as a solvent without any catalyst. The reaction offers potent biologically active spirooxindole fused with indole and pyridine heterocycles in good to excellent yield (69-94%) with higher diastereoselectivity. These synthesized compounds (4a-x) were screened for anticancer activity using A549, HepG-2, and SKOV-3 cancer cell lines using the MTT assay. Among all the screened compounds, 4u and 4w displayed substantial cytotoxic activity against HepG-2 cells at less than 10 μg mL−1. Molecular docking studies with the Bcl-2 and ALK receptor revealed that the higher binding energy was observed for 4u and 4w, and 4c and 4o with a value of −6.56 and −8.41, −6.73, and −7.14 kcal mol−1, respectively. Considering all the data, compounds 4u and 4w, 4c and 4o possess potent anticancer activity against respective receptors and can be the promising lead compounds for cancer drug discovery. © 2022 The Royal Society of Chemistry

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IITH Creators:

IITH Creators	ORCiD

Item Type:

Article

Additional Information:

Sivakalai Mayakrishnan acknowledges the CSIR, India, for providing a Senior Research Fellowship (Grant No. 31/6(422)/2017-EMR-I) and TATA-CSIR-Open Source Drug Discovery (TCOF). Uma Maheswari Narayanan acknowledges Major Lab Project (MLP-12 Organic & Bioorganic Chemistry) CSIR-CLRI toward purchasing chemicals. PY thanks Science and Engineering Research Board (SERB) New Delhi, India (GPP 0334 –Ref. No: EEQ/2017/000161) for financial support.

Uncontrolled Keywords:

Anti-cancer agents; Anticancer activities; Anticancer drug; Heterocycles; In-silico; Medicinal chemistry; Multicomponents; Natural products; Pyrrolidines; Silico studies

Subjects:

Chemistry

Divisions:

Department of Chemistry

Depositing User:

. LibTrainee 2021

Date Deposited:

11 Oct 2022 11:45

Last Modified:

11 Oct 2022 11:45

URI:

http://raiithold.iith.ac.in/id/eprint/10893