Chitosan IR806 dye-based polyelectrolyte complex nanoparticles with mitoxantrone combination for effective chemo-photothermal therapy of metastatic triple-negative breast cancer

Alvi, S.B. and Rajalakshmi, P.S. and Jogdand, A.B. and Nazia, B. and Bantal, V. and Rengan, Aravind Kumar (2022) Chitosan IR806 dye-based polyelectrolyte complex nanoparticles with mitoxantrone combination for effective chemo-photothermal therapy of metastatic triple-negative breast cancer. International Journal of Biological Macromolecules, 216. pp. 558-570. ISSN 0141-8130

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Abstract

Chemo-photothermal therapy is one of the emerging therapies for treating triple-negative breast cancer. In this study, we have used ionotropic gelation method to fabricate chitosan and IR806 dye-based polyelectrolyte complex (CIR-PEx) nanoparticles. These nano-complexes were in size range of 125 ± 20 nm. The complexation of IR 806 dye with chitosan improved photostability, photothermal transduction, and showed excellent biocompatibility. Cancer cells treated with CIR-PEx NPs enhanced intracellular uptake within 5 h of incubation and also displayed mitochondrial localization. With the combination of CIR-PEx NPs and a chemotherapeutic agent (i.e., mitoxantrone, MTX), a significant decline in cancer cell viability was observed in both 2D and 3D cell culture models. The chemo-photothermal effect of CIR-PEx NPs + MTX augmented apoptosis in cancer cells when irradiated with NIR light. Furthermore, when tested in the 4 T1-tumor model, the chemo-photothermal therapy showed a drastic decline in tumor volume and inhibited metastatic lung nodules. The localized hyperthermia caused by photothermal therapy reduced the primary tumor burden, and the chemotherapeutic activity of mitoxantrone further complemented by inhibiting the spread of cancer cells. The proposed chemo-photothermal therapy combination could be a promising strategy for treating triple-negative metastatic breast cancer. © 2022 Elsevier B.V.

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IITH Creators:

IITH Creators	ORCiD
Rengan, Aravind Kumar	https://orcid.org/0000-0003-3994-6760

Item Type:

Article

Additional Information:

AKR would like to acknowledge funding agencies CRG/2020/005069 , BT48 NNT/28/1836/2017 , MHRD-IMPRINT- 1 & 2 (4291, 00275 ), ICMR grant No35/1/2020-GIA/Nano BMS , Govt. of India, ICMR CoE for their generous financial support. SBA would like to thank the CSIR for SRF as financial support ( 09/1001(0044)/2019-EMR-1 ). RJ would like to acknowledge the UGC ( 938/(OBC ) (CSIR-UGC NET Dec.2016)) for financial support. AJ would like to acknowledge the UGC ( 201516-RGNF-2015-17-SC-MAH-14156 ).

Uncontrolled Keywords:

Breast cancer; Chemo-photothermal therapy; Chitosan nanosystems; IR806; Metastasis; Mitoxantrone

Subjects:

Biomedical Engineering

Divisions:

Department of Biomedical Engineering

Depositing User:

. LibTrainee 2021

Date Deposited:

11 Oct 2022 09:31

Last Modified:

11 Oct 2022 09:31