A plasmon-enhanced fluorescent gold coated novel lipo-polymeric hybrid nanosystem: synthesis, characterization and application for imaging and photothermal therapy of breast cancer

Appidi, Tejaswini and P.S, Rajalakshmi and Chinchulkar, Shubham A. and Pradhan, Arpan and Begum, Hajira and Shetty, Veeresh and Srivastava, Rohit and Ganesan, Prabhusankar and Rengan, Aravind Kumar (2022) A plasmon-enhanced fluorescent gold coated novel lipo-polymeric hybrid nanosystem: synthesis, characterization and application for imaging and photothermal therapy of breast cancer. Nanoscale, 14 (25). pp. 9112-9123. ISSN 2040-3364

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Abstract

This study reports a hybrid lipo-polymeric nanosystem (PDPC NPs) synthesized by a modified hydrogel-isolation technique. The ability of the nanosystem to encapsulate hydrophilic and hydrophobic molecules has been demonstrated, and their enhanced cellular uptake has been observed in vitro. The PDPC NPs, surface coated with gold by in situ reduction of chloroauric acid (PDPC-Au NPs), showed a photothermal transduction efficacy of ∼65%. The PDPC-Au NPs demonstrated an increase in intracellular ROS, triggered DNA damage and resulted in apoptotic cell death when tested against breast cancer cells (MCF-7). The disintegration of PDPC-Au NPs into smaller nanoparticles with near-infrared (NIR) laser irradiation was understood using transmission electron microscopy imaging. The lipo-polymeric hybrid nanosystem exhibited plasmon-enhanced fluorescence when loaded with IR780 (a NIR dye), followed by surface coating with gold (PDPC-IR-Au NPs). This paper is one of the first reports on the plasmon-enhanced fluorescence within a nanosystem by simple surface coating of Au, to the best of our knowledge. This plasmon-enhanced fluorescence was unique to the lipo-polymeric hybrid system, as the same was not observed with a liposomal nanosystem. The plasmon-enhanced fluorescence of PDPC-IR-Au NPs, when applied for imaging cancer cells and zebrafish embryos, showed a strong fluorescence signal at minimal concentrations of the dye. The PDPC-IR-Au NPs were also applied for photothermal therapy of breast cancer in vitro and in vivo, and the results depicted significant therapeutic benefits. © 2022 The Royal Society of Chemistry.

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IITH Creators:

IITH Creators	ORCiD
Rengan, Aravind Kumar	https://orcid.org/0000-0003-3994-6760

Item Type:

Article

Additional Information:

The authors would like to thank the MOE IMPRINT (4291), CRG/2020/005069, DST/TDT/AMT/2017/227, BME ID project, ICMR grant no 35/1/2020-GIA/Nano BMS, Govt. of India, for research funding. The authors would like to acknowledge the generous financial support from Vasudha Foundation, Hyderabad, India. The author TA would like to thank DST, Govt. of India, for the DST-Inspire fellowship (IF160291). The author, RJ, would like to thank UGC, Govt of India, for her fellowship UGC (938/(OBC)(CSIR-UGC NET DEC.2016). The authors would like to acknowledge Lipoid, Germany, for providing DOPS-Na gift samples. The graphical abstract was prepared using Biorender.com.

Uncontrolled Keywords:

Breast Cancer; Enhanced fluorescence; Gold-coated; Hybrid nanosystem; In-vitro; Isolation techniques; Photothermal therapy; Polymeric hybrids; Surface coatings; Synthesised

Subjects:

Biomedical Engineering

Divisions:

Department of Biomedical Engineering

Depositing User:

. LibTrainee 2021

Date Deposited:

08 Oct 2022 09:10

Last Modified:

08 Oct 2022 09:10

URI:

http://raiithold.iith.ac.in/id/eprint/10856