3D imaging and quantification of PLL coated fluorescent ZnO NP distribution and ROS accumulation using laser scanning confocal microscopy

Dey, Aishee and Gare, Suman and Swain, Sarpras and Bhattacharya, Proma and Dhyani, Vaibhav and Giri, Lopamudra and et al, . (2022) 3D imaging and quantification of PLL coated fluorescent ZnO NP distribution and ROS accumulation using laser scanning confocal microscopy. AIChE Journal, 68 (9). ISSN 0001-1541

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Abstract

Investigations on nanomedicine involve conventional two dimensional (2D) imaging techniques for observing the nanoparticle internalization at a single time point where various phases of internalization can be overlooked. In contrast, three dimensional (3D) imaging of fluorescent nanoparticles with anticancer potential can be used for obtaining the time course of cellular retention of particles, and cells can be followed for days. This article demonstrates the application of laser scanning confocal microscopy to quantify poly-l-lysine coated fluorescent ZnO nanoparticle retention and reactive oxygen species (ROS) generation using volumetric imaging. Synthesis of these particles allows monitoring of ROS formation, internalization, and cytotoxicity using the same imaging platform that offers an advantage over measurement using various instruments. PLL-coated ZnO particles' ability to induce a significant reduction in cell-viability suggests its potential as a therapeutic agent. The proposed framework opens up a new avenue for investigating mechanistic aspects of ZnO adsorption and the evaluation of therapeutic efficiency. © 2022 American Institute of Chemical Engineers.

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IITH Creators:

IITH Creators	ORCiD
Giri, Lopamudra	http://orcid.org/0000-0002-2352-7919

Item Type:

Article

Additional Information:

The authors acknowledge the research facilities provided by the Indian Institute of Technology Kharagpur, India, and the Indian Institute of Technology Hyderabad, India. The authors also acknowledge Mr. K. Venkateswarlu from Indian Institute of Technology Hyderabad, India for his guidance in writing the article and Mr. Nilanjan Dutta from Indian Institute of Technology Madras, India for his valuable contribution in statistical data analysis.

Uncontrolled Keywords:

3D live-cell imaging; fluorescent ZnO particle; laser scanning confocal microscope; reactive oxygen species; therapeutic efficiency

Subjects:

Chemical Engineering

Divisions:

Department of Chemical Engineering

Depositing User:

. LibTrainee 2021

Date Deposited:

07 Oct 2022 09:45

Last Modified:

07 Oct 2022 09:45