Single-cell transcriptomics and cell-specific proteomics reveals molecular signatures of sleep

Jha, Pawan K. and Valekunja, Utham K. and Ray, Sandipan and et al, . (2022) Single-cell transcriptomics and cell-specific proteomics reveals molecular signatures of sleep. Communications Biology, 5 (1). pp. 1-16. ISSN 2399-3642

Text
communications_biology.pdf - Published Version
Available under License Creative Commons Attribution.
Download (7MB)

Abstract

Every day, we sleep for a third of the day. Sleep is important for cognition, brain waste clearance, metabolism, and immune responses. The molecular mechanisms governing sleep are largely unknown. Here, we used a combination of single-cell RNA sequencing and cell-type-specific proteomics to interrogate the molecular underpinnings of sleep. Different cell types in three important brain regions for sleep (brainstem, cortex, and hypothalamus) exhibited diverse transcriptional responses to sleep need. Sleep restriction modulates astrocyte-neuron crosstalk and sleep need enhances expression of specific sets of transcription factors in different brain regions. In cortex, we also interrogated the proteome of two major cell types: astrocytes and neurons. Sleep deprivation differentially alters the expression of proteins in astrocytes and neurons. Similarly, phosphoproteomics revealed large shifts in cell-type-specific protein phosphorylation. Our results indicate that sleep need regulates transcriptional, translational, and post-translational responses in a cell-specific manner.

[error in script]

IITH Creators:

IITH Creators	ORCiD
Ray, Sandipan	http://www.orcid.org/0000-0002-9960-5768

Item Type:

Article

Additional Information:

A.B.R. acknowledges funding from the Perelman School of Medicine, University of Pennsylvania, the Institute for Translational Medicine and Therapeutics (ITMAT) at the University of Pennsylvania. This work was supported also by NIH DP1DK126167 and R01GM139211 (A.B.R).

Uncontrolled Keywords:

GENE-EXPRESSION,RAT-BRAIN,DEPRIVATION

Subjects:

Others > Biotechnology

Divisions:

Department of Biotechnology

Depositing User:

. LibTrainee 2021

Date Deposited:

02 Sep 2022 06:44

Last Modified:

05 Sep 2022 09:01