3D Bioprinting of Multi-Material Decellularized Liver Matrix Hydrogel at Physiological Temperatures

Khati, Vamakshi and Ramachandraiah, Harisha and Pati, Falguni and et al, . (2022) 3D Bioprinting of Multi-Material Decellularized Liver Matrix Hydrogel at Physiological Temperatures. Biosensors, 12 (7). pp. 1-18. ISSN 2079-6374

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Abstract

Bioprinting is an acclaimed technique that allows the scaling of 3D architectures in an organized pattern but suffers from a scarcity of appropriate bioinks. Decellularized extracellular matrix (dECM) from xenogeneic species has garnered support as a biomaterial to promote tissue-specific regeneration and repair. The prospect of developing dECM-based 3D artificial tissue is impeded by its inherent low mechanical properties. In recent years, 3D bioprinting of dECM-based bioinks modified with additional scaffolds has advanced the development of load-bearing constructs. However, previous attempts using dECM were limited to low-temperature bioprinting, which is not favorable for a longer print duration with cells. Here, we report the development of a multi-material decellularized liver matrix (dLM) bioink reinforced with gelatin and polyethylene glycol to improve rheology, extrudability, and mechanical stability. This shear-thinning bioink facilitated extrusion-based bioprinting at 37 °C with HepG2 cells into a 3D grid structure with a further enhancement for long-term applications by enzymatic crosslinking with mushroom tyrosinase. The heavily crosslinked structure showed a 16-fold increase in viscosity (2.73 Pa s−1) and a 32-fold increase in storage modulus from the non-crosslinked dLM while retaining high cell viability (85–93%) and liver-specific functions. Our results show that the cytocompatible crosslinking of dLM bioink at physiological temperatures has promising applications for extended 3D-printing procedures. © 2022 by the authors.

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IITH Creators:

IITH Creators	ORCiD
Pati, Falguni	http://orcid.org/0000-0002-3588-1800

Item Type:

Article

Additional Information:

This research was partly funded by Swedish Research Council, grant number 2015-05378, and European Commission through the FP7 project CanDo, grant number 610472. GG acknowledges funding from the Swedish Research Council, grant number 2019-05170.

Uncontrolled Keywords:

bioprinting at physiological temperatures; cytocompatible crosslinking; decellularized liver matrix bioink; robust bioink; viscoelasticity

Subjects:

Biomedical Engineering

Divisions:

Department of Biomedical Engineering

Depositing User:

. LibTrainee 2021

Date Deposited:

01 Sep 2022 10:28

Last Modified:

01 Sep 2022 10:28