In-vivo Single-Molecule Imaging in Yeast: Applications and Challenges

Podh, Nitesh Kumar and Paliwal, Sheetal and Dey, Partha and Das, Ayan and Morjaria, Shruti and Mehta, Gunjan (2021) In-vivo Single-Molecule Imaging in Yeast: Applications and Challenges. Academic Press.

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Abstract

Single-molecule imaging has gained momentum to quantify the dynamics of biomolecules in live cells, as it provides direct real-time measurements of various cellular activities under their physiological environment. Yeast, a simple and widely used eukaryote, serves as a good model system to quantify single-molecule dynamics of various cellular processes because of its low genomic and cellular complexities, as well as its facile ability to be genetically manipulated. In the past decade, significant developments have been made regarding the intracellular labeling of biomolecules (proteins, mRNA, fatty acids), the microscopy setups to visualize single-molecules and capture their fast dynamics, and the data analysis pipelines to interpret such dynamics. In this review, we summarize the current state of knowledge for the single-molecule imaging in live yeast cells to provide a ready reference for beginners. We provide a comprehensive table to demonstrate how various labs tailored the imaging regimes and data analysis pipelines to estimate various biophysical parameters for a variety of biological processes. Lastly, we present current challenges and future directions for developing better tools and resources for single-molecule imaging in live yeast cells. © 2021 Elsevier Ltd

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IITH Creators:

IITH Creators	ORCiD
Mehta, Gunjan	https://orcid.org/0000-0003-2896-3039

Item Type:

Other

Additional Information:

This work is supported by the Har-Govind Khorana Innovative Young Biotechnologist Award (BT/13/IYBA/2020/10) and the Ramalingaswami Fellowship to GM from the Department of Biotechnology, Govt. of India, and the seed grant from IIT Hyderabad to GM. We acknowledge Dr. Tatiana Karpova and Dr. David Ball (National Institutes of Health, USA) to share unpublished data shown in Figure 2. The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Uncontrolled Keywords:

binding kinetics; fluorescence microscopy; HILO; single-molecule imaging/tracking; yeast

Subjects:

Others > Biotechnology

Divisions:

Department of Biotechnology

Depositing User:

. LibTrainee 2021

Date Deposited:

29 Aug 2022 10:53

Last Modified:

29 Aug 2022 10:53