Efficient separation and quantification of methyl palmitate and methyl oleate in biodiesel mixture using reverse-phase high performance liquid chromatography

Mandari, Venkatesh and Devarai, Santhosh Kumar (2021) Efficient separation and quantification of methyl palmitate and methyl oleate in biodiesel mixture using reverse-phase high performance liquid chromatography. Indian Chemical Engineer. pp. 1-9. ISSN 0019-4506

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Abstract

The excessive utilization of petroleum resources leads to global warming, crude oil price fluctuations, and fast depletion of petroleum reserves. Biodiesel (Fatty Acid Methyl Ester, FAME) has gained importance as a clean and renewable energy source over the last few years. It is produced by reacting vegetable oils and short-chain alcohols via transesterification/esterification reaction. The properties of biodiesel depend on the vegetable oils’ fatty acid composition due to the saturated and unsaturated fatty acid content variation. During the FAME quantification analysis, separation of methyl palmitate (MeP) and methyl oleate (MeO) are major challenges in the C18 column in High Performance Liquid Chromatography (HPLC). These components are difficult to quantify as they elute simultaneously by forming an overlapping peak. A new reverse-phase HPLC separation method with C18 column has been developed to efficiently separate and quantify FAMEs derived from palm oil. UV-VIS detector was used at 205 nm. The FAMEs separation was achieved using ternary mobile phase solvents acetonitrile, water, and acetone with the composition of 62%, 33%, and 5% in an isocratic flow with 2.20 mL/min flowrate and 45 °C column temperature. Methyl esters were analyzed with satisfactory linearity (r 2 >0.99), repeatability (relative standard deviation <2%), and sensitivity (limit of quantification). © 2021 Indian Institute of Chemical Engineers.

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IITH Creators:
IITH CreatorsORCiD
Devarai, Santhosh Kumarhttps://orcid.org/0000-0001-5298-0926
Item Type: Article
Uncontrolled Keywords: Biodiesel; fatty acid methyl esters; HPLC; methyl oleate; methyl palmitate
Subjects: Chemical Engineering
Divisions: Department of Chemical Engineering
Depositing User: . LibTrainee 2021
Date Deposited: 19 Aug 2022 04:16
Last Modified: 19 Aug 2022 04:16
URI: http://raiithold.iith.ac.in/id/eprint/10216
Publisher URL: http://doi.org/10.1080/00194506.2021.1997652
OA policy: https://v2.sherpa.ac.uk/id/publication/5340
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